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Primary hosting computer server companies used for immunoprecipitation and Western blotting were as follows: anti-NRBF2 (Cell Signaling Technology), anti-Vps34 for immunoprecipitation 24anti-Vps34 for Western blotting 25anti-Vps15 18anti-Beclin-1 (BD Biosciences), anti- Atg14L (MBL International), anti-UVRAG (Cell Signaling Technology), anti-LC3 (Cell Remote desktop connection windows 2000 server download Technology), anti-p62 (MBL International), anti-V5 for immunoprecipitation (Thermo Scientific); anti-V5 for Western blotting (Life Technologies), anti-FLAG (Sigma-Aldrich); anti-actin (Sigma-Aldrich), anti-GAPDH (glyceraldehyde-3-phosphate dehydrogenase; MBL International) and anti-(rabbit IgG) (Jackson ImmunoResearch). I would recommend Ramnode (affiliated) as a VPS provider. The HindIII site that underwent a change in its position is marked by an asterisk. Punctae were counted manually and the data are pooled from three separate experiments with 100 cells counted per condition in each experiment.

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